Validation of a Liquid Chromatography Method for the 1 Simultaneous Quantification of Ochratoxin a and Its
نویسندگان
چکیده
12 A validated high-performance liquid chromatography (HPLC) method with 13 fluorescence detection for the simultaneous quantification of ochratoxin A (OTA) and 14 its analogues (ochratoxin B (OTB), ochratoxin C (OTC) and methyl ochratoxin A 15 (MeOTA)) in red wine at trace levels is described. Before their analysis by HPLC-FLD, 16 ochratoxins were extracted and purified with immunoaffinity columns from 50 mL of 17 red wine at pH = 7.2. Validation of the analytical method was based on the following 18 parameters: selectivity, linearity, robustness, limits of detection and quantification, 19 precision (within-day and between-day variability), recovery and stability. The limits of 20 detection (LOD) in red wine were established at 0.16, 0.32, 0.27 and 0.17 ng L
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